Dear Editor,
Recent study shows that the prime editing system fusing the Cas9 nickase and reverse transcriptase could perform all types of gene modifications, including base substitutions (transitions and transversions), small insertions, and deletions, without requiring donor DNA or double-strand breaks (DSBs) (Anzalone et al., 2019). Despite the accuracy and versatility, the efficiency of the prime editor (PE) is often insufficient, which limits its broad applications.